Assignment
for Friday's class:
Go through today's blast slides and think about how you will transfer files back and forth from the cluster.
Assignment for Monday
Complete take home exam #3 (due on Monday)
Continuation from class 10
blast and commandline blast
(the slides cotain links that only become accessible, after you switched to presentation mode)
ELSE
Where to get genomes:
ftp://ftp.ncbi.nih.gov/genomes/Bacteria/
(check out different files (.faa .fna .ffn .ptt) using
A. veronii
and
A. salmonicida
as example)
discuss blastall, example analysis for pairwise genome comparison is
here
(discuss %identity, alignment lengthd, #of identical aa)
example of what one could do with this:
(perl script to extract top scoring hits is
here
)
(perl script to replace gi number with position on the genome is
here
)
(perl script to make gnuplot is
here
, output is
here
)
sequence space