Discussion of homework:

Discussion of lab?

Interactions between aa and DNA: THe question in that lab asks for base pairs, i.e. part of the DNA
Lys 340: magenta, Glu366: red

1LWS_G17_chainB
C22 chainC

 

Fridays for Future & Computerlab

Definitions of life? Gaia, Medea (daisyworld versus snowball Earth)

Daisyworld model (simulation here or here), in which black and white Daisies stabilize the climate of the model planet. The release of DMS heat stressed algae creates a Daisyworld like feed back loop, because it acts as nucleating agent in cloud formation.

Snowball Earth (movie here) (feed forward instead of feed back - question: What prevents the Earth from oscilating back and forth between green house and ice house?)

 

See posted answers on class home page!

Which of the following statements is correct:

  1. All complex sequences that show significant similarity in a pairwise sequence comparison are homologous
  2. All homologous sequences show significant similarity in a pairwise sequence comparison.
  3. Both of the above statements are correct


Which of the following is NOT considered strong evidence for homology?

  1. The arrangement of secondary structure elements in space is the same or very similar.
  2. Significant primary sequence similarity
  3. Significant primary sequence identity
  4. Identical function
  5. None of the above

Selection for function can preserve sequence similarity in the pairwise comparison of homologous proteins, across domains separated by how many years of independent evolution?

  1. Thousands
  2. Millions
  3. Billions
  4. Limited only by how long life has existed
  5. All of the above

Which of the following is NOT part of the explanation for how complex functional molecules were assembled, despite the vastness of protein space?

  1. Gaia directs protein evolution, through negative feedback loops, to the correct region of protein space.
  2. There are multiple unrelated solutions for the same functionality, exemplified by the fact that there are non-homologous enzymes inhabiting completely different regions of protein space with the same function.
  3. An exact function does not need to be hit upon, because natural selection can take a protein with limited function and make it better.
  4. Similar structures have similar function, so there are entire regions of protein space occupied by homologs that all function equally well, or nearly so.
  5. Protein space is made slightly smaller by removing all of the possibilities that cannot be synthesized or they will clog up the ribosome

 

Go through coral of life ppt slides.

Slides on Mutual Aid versus(?) Natural Selection here

 

 

How old is life on Earth?

  • The Earth is about 4.6 Ga old, but no crustal rocks has survived from that time. The oldest rocks are no older than 4.0 Ga. But zircon crystals dated to 4.4 billion years BP are found in rocks, are considered evidence for Earth's crust having had formed and being in contact with water already 4.4 billion years BP (here and here)

Morphological Fossil Evidence:
  • For about a decade the oldest microfossils were considered to be about 3.5 Ga old (see here). The fossils (as interpreted by Bill Schopf) look like "modern" Cyanobacteria. Compare the time to to molecular trees of life: Is this a problem? However, the evidence for these fossils was questioned.

  • 3.2Ga old filamentous fossils, probably of thermophilic chemotrophic prokaryotes (Rasmussen, 2000)

  • 1.8Ga old fossils from Gunflint formation: iron-loving bacteria and cyanobacteria
Biological Signature Evidence (examples):
  • Oldest geological evidence for life - 3.8 Ga ago - is based on 13C discrimination (carbon derived from living systems often have lower delta 13C values than inorganic carbonates) [here]. The rocks are from Akilia island off the coast of Greenland, and severely altered by metamorphism. However, recently the evidence for that was reassessed.
  • 3.7 billion year of rocks from the Isua formation in Greenland contain structures described as stromatholites. here in NYT, report in Nature
  • The amount of carbon in the Issua formation (and its discrimination against agains 13C) is interpreted by Minik Rosing to indicate a highly productive biosphere. (See Minik's presentation at the 2011 ISSOL meeting. Especially slides 6 ff are interesting - the handwritten slides were written with the sedimentary rocks - containing lots of graphite.)

  • 2.7Ga old: probable biomarkers of cyanobacteria and of eukaryotes (Roger Summons, Roger Buick and Jochen Brocks)

See Olga's Timeline of the Universe here, see the toilet-paper timeline of Earth here (the dates are off, but the relative ditances are nicely demonstated).

 

If time, go through slides on high temperature origin of life and the late heavy bombardment